CD-1 Hepatocytes plateable

We offer hepatocytes in plateable form isolated from various species and breed, ensuring high viability and prolonged healthy culture duration. These cells undergo comprehensive characterization for transporter activity, enzyme activity, and induction potential to meet the highest quality standards. Choose our hepatocytes for robust and reliable results in your research and drug developmental studies.

Available formats:
proliferating: ≥ 500000 viable cells shipped in appropriate growth media (T25).
Cryopreserved: Cryogenic vial containing ≥ 500000 viable cells

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+ MSDS
+ PI

General Description Specifications Certificate Of Analysis Technical Library

In drug development projects, there is a significant concern about finding models for drug-to-drug interactions (DDI) with clinical relevance. Plateable hepatocytes, specialized primary liver cells, are derived from various species according to customer requirements. These cells possess a unique ability to adhere and grow on culture plates or dishes, making them indispensable tools for drug development, toxicology, liver-related studies, and disease modeling.

At Kosheeka, we ensure that these hepatocytes are thoroughly tested for high viability and sustained healthy culture duration. The possess induction qualifications, making them highly valuable for DDI studies. These cells undergo comprehensive characterization for transporter activity and enzyme activity, focusing on major mechanisms involved in drug interactions.

The featured characteristics of these hepatocytes include confluence monolayer, distinct cobblestone appearance, and inducibility of enzyme activity for CYP3A4, CYP2B6, and CYP1A2. We also analyze their basal metabolism for eight CYPs, SULT, UGT, and aldehyde oxidase. Additionally, we pretest these cells for long-term culture, spheroid formation, and compatibility with 96-well plates.

Choose our plateable hepatocytes to enhance the clinical relevance of your drug development projects and ensure accurate predictions of drug interactions for better research outcome.

Product Category	Mus musculus, Mouse CD-1
Product Type	Adherent population of cells
Derived From	Liver tissues
Cell Morphology	Cobble stone morphology with tight junctions
Culture & Growth Properties	Adherent population of cells
Passage No.	P1
Mycoplasma	Detected on demand
Hepatitis B	Negative
Hepatitis C	Negative
HIV-1	Negative
Aerobic & Anaerobic Bacteria	Negative after 7 days of culture
Positive for	Transporter qualified
Negative for

The plateable hepatocytes are cultured using specialized media and optimized culture conditions that support their growth. The medium is supplemented with growth factors and nutrients to maintain liver-specific functions and keep them viable.

Our QC tests have shown that these cells can be cultured up to passage 5. However, the actual culture duration may vary depending on the factors such as culture conditions, media used, and handling techniques.

These hepatocytes are commonly cultured using Wiliam’s E medium supplemented with 10% FBS. Additionally, specific growth factors like hepatocyte growth factors are added at the optimal concentration to the culture.

Yes, these plateable hepatocytes can be cryopreserved for long term storage; allowing for future use for various experiments.

For spheroid formation, hematopoietic culture media supplemented with 20% FBS and 25 mM HEPES is recommended. No plating media is required during spheroid seeding.

Yes, hepatocytes should be cultured on a collagen coating to ensure strong adherence and proper growth.

Yes, the optimized protocols typically require around 5000 cells per well in a 6-well plate. Plating more cells may lead to poor attachments at higher densities.

To avoid cellular compaction in the pellet and difficulties in resuspending cells, it is advised to use a 50 ml round bottom for pelletting.

A detailed certificate of analysis will be provided with the products. However, if you require raw data for publication purposes, a non-disclosure agreement must be signed, acknowledging Kosheeka as your cell supplier.

Yes, we offer customizations options to meet your specific requirements, including hepatocytes from particular species and breeds, with the desired quality control tests.