Available formats: ≥ 2.5 x 106/ ml. cryopreserved in suitable medium
Product Category
Homo Sapiens, Human
Product Type
Adherent population of cells
Derived From
Peripheral Blood
Cell Morphology
Round, light emitting cells
Culture & Growth Properties
Adherent population with elongated flap like structures
Passage No.
P2
Mycoplasma
Detected on demand
Hepatitis B
Negative
Hepatitis C
Negative
HIV-1
Negative
Aerobic & Anaerobic Bacteria
Negative after 7 days of culture
Positive for
CD8
Negative for
CD 34
These are the type of T cells, also known as CD8+ cells with the ability to regulate immune response in the body and fight against invading pathogens. They also regulate production of abnormal cells like cancer cells. There are several sources of cytotoxic T cells like bone marrow, peripheral blood and umbilical cord blood, which can be easily exploited to extract cells.
Bone marrow is the most potent source of cytotoxic T cells, where the immature T cells or naïve cells are produced. The choice of source depends upon your research demand, if your study requires immature T cells, bone marrow is the best match. However, if mature T cells, positive for CD8+ is required, they can be easily isolated from peripheral blood, without much ethical concerns.
Yes, cytotoxic T cells can be expanded in culture, known as T cell culture or lymphocyte culture. For expansion a specific T lymphocyte expansion media is suggested with either serum free option or with 2 % autologous human serum. The expansion of cytotoxic T cells is modified with the use of different cytokines, growth factors, co-stimulation as well as genetic modifications, depending upon number of applications.
Although, specific method for isolation of cytotoxic T cells depends upon the research applications, some of the general methods used for the isolation of T cells are density gradient centrifugation, magnetic bead separation, fluorescence activated cell sorting, etc. We generally isolate cells from a pool of healthy peripheral blood donor, obtained through leukapheresis and obtaining T lymphocytes from a pool of mononuclear cells; the cells are further purified using positive magnetic selection for different applications. However, we emphasize you to define your specifications and experimental requirements, so that we can offer the quality cells.
Each cell expresses a different subset of proteins, while CD8+ cell expresses CD8, the CD4+ T cell expresses the CD4 subset. Moreover, both the cells functions very differently, while CD8+ T cells are primarily involved in identifying foreign antigen and tumorigenic cell and promoting their phagocytosis and/or apoptosis, the CD4+ T cells are primarily involved in regulating immune response through secretion of a cocktail of cytokines and activating immune cells.
The CD8+ T cells have a specific receptor known as T cell receptor, which identifies a major histocompatibility complex formed with a particular foreign antigen. Upon identification, the TCR binds with the major histocompatibility complex on the cellular surface and activates intracellular signalling pathways, in order to initiate their phagocytosis and apoptosis.
The CD8+ T cells are activated upon recognition of specific major histocompatibility complexes presented on the surface of invading foreign antigen as well as abnormal cells. These antigens are presented to the T cells by specialized cells, called antigen-presenting cells (APCs), like dendritic cells. Once activated by the CD8+ cells undergo clonal expansion and differentiate into effector cells that can recognize and get rid of the targeted antigens.
The culture of CD8+ T cells have been extensively used in multiple research and development projects, including the development of immunotherapies for cancer and infectious diseases. The cells are also used in the study of autoimmune disorders, and the generation of engineered T cells for adoptive cellular therapies. Additionally, CD8+ T cells cultures can be used to test the efficacy and safety of drugs development and toxicity analysis.
Certain cells like CD8+ T cells are difficult to expand in the culture due to the requirement of addition of certain cocktail of growth factor or cytokines to promote cellular expansion. Additionally, these cytokines are highly sensitive and specific about their concentration as well as other optimum culture conditions; if not maintained properly, they can initiate cellular apoptosis. Moreover, these cells are also very sensitive about the culture hand, any wrong handling can create contamination in the culture.
The transportation of CD8+ T cells can either be in adherent form or in cryopreserved form in dry ice. Kosheeka transports CD8+ T cells with unique technology through which cells are being shipped at room temperature to maintain the viability and function of the cells during transit. We encourage proper quality control of the cells being shipped for viability, characterization and functional contribution.
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