Human Bone Marrow-Derived Mesenchymal Stem Cells

Available Formats:

Proliferating: ≥500000 viable cells shipped in appropriate growth medium (T25 Flask).
Cryopreserved: A cryogeneic vial containing ≥500000 cells

General Description Specifications Certificate Of Analysis Technical Library

Mesenchymal stem cells, also commonly known as mesenchymal stromal cells obtained from bone marrow are multipotent cells with the ability to differentiate into cells of various mesenchyme origins. These cells can be differentiated in vitro into different cell types, including osteoblasts, chondrocytes, and adipocytes. Due to their distinct degrees of potency, these cells are being extensively studied for their applications in regenerative medicines and tissue engineering. These cells are also widely studied for cosmetic applications, drug discovery, toxicity studies, and in-vitro disease modeling.Kosheeka Normal Human Bone Marrow-Derived Mesenchymal Stem cells are harvested from human bone marrow with informed consent; from individual donors. These cells are tested for their viability, and characteristic stemness marker’s in vitro ability to differentiate into adipocytes, chondrocytes, and osteocytes.

Product Category	Homo Sapiens, Human
Product Type	Adherent cells
Derived From	Bone Marrow
Cell Morphology	Elongated, spindle-shaped
Culture & Growth Properties	Adherent, proliferating population
Passage No.	Customized
Mycoplasma	Detected on demand
Hepatitis B	Negative
Hepatitis C	Negative
HIV-1	Negative
Aerobic & Anaerobic Bacteria	Negative after 7 days of culture
Positive for	CD 105, CD 90
Negative for	CD 45, 34

Kosheeka’s human mesenchymal stem cells are isolated from bone marrow, which is withdrawn from bilateral punctures of the posterior iliac crests of normal volunteers. Please see the product information sheet for more information.

No, mesenchymal stem cells are a strongly adherent population of fibroblasts that don’t need any extracellular matrix for proliferation and cellular expansion.

Mesenchymal stem cells isolated from various sources exhibit the basic difference in potency. Mesenchymal stem cells isolated from umbilical cord tissue are pluripotent in nature as they are formed at a very early stage of fetal development; hence, they can be differentiated into cells of neuronal origin. Whereas mesenchymal stem cells isolated from bone marrow are multipotent in nature, for being isolated fully grown adult committed tissue.

The cumulative population doublings are calculated using the seeding density and harvesting density from each type of culture vessel. The cell doubling time is calculated using the formula Doubling Time = Duration * log (2)/ log [Final concentration/Initial Concentration]

No, these cells are isolated from mononuclear fraction; whereas mononuclear cells are isolated using differential centrifugation technique.

Since these are mesenchymal stem cells isolated from adipose tissue, they can be passaged up to number 10 in the culture without losing their characteristics. However, with multiple freeze-thaw processes, cells can lose their morphology and stemness.

It varies from lot to lot depending on donor demographics like age, sex, health condition, family history, size of the tissue, etc. Typically, around 1 T25 flask can give you about 500000 cells or more.

We have standardized our protocol for each primary cell type using different media and growth factor combinations for rapid growth potential. Although, we suggest cells be grown in our own media; however, media from any reputed manufacturer like Gibco can be used depending upon internal standardized protocol.

Each tissue collection is done with informed consent from a healthy donor. The donor is tested for infectious markers like HIV-1, HCV, HBsAg, HBcAg, etc. Once the cells are isolated, a batch is tested for gram-positive and gram-negative bacteria. The cultures are also assessed for mycoplasma. The cells are characterized by specific mesenchymal stem cells markers like CD 105, and CD 90.