Human Peripheral Blood-Derived Cytotoxic T-Cells

The human peripheral blood derived cytotoxic T cells, also known as the CD8+ T cells and cytotoxic T cells, are a kind of T lymphocytes that play a very important role in protecting us from invading infectious agents and cancer through regulation of immune system. These cells are a subset of T lymphocytes, the key […]

Available formats: ≥ 2.5 x 106/ ml. cryopreserved in suitable medium

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General Description Specifications Certificate Of Analysis Technical Library

Most cytotoxic T cells express T-cell receptors (TCRs), which are helpful in recognizing a specific glycoprotein and an antigen called as CD8, binding with the constant portion of the class I MHC. Upon binding, these cells release certain cellular cytotoxins like perforin, granzymes as well as granulysin that are responsible for triggering cascade of signalling reactions promoting apoptosis. This important property makes them important tool for understanding pathophysiology of many viral diseases including hepatitis B and inflammatory diseases like rheumatoid arthritis. Moreover, these cells have also shown promising results in the treatment of certain cancers like melanoma, leukaemia, etc.

The peripheral blood derived cytotoxic T cells are obtained from patient’s own samples and can be expanded upon activation using a cocktail of cytokines and other growth factors. Kosheeka recommends use of IL-2 to activate T cells that are further purified based on the expression of surface marker CD8, which is the hallmark of cytotoxic T cells. The final product is further tested for purity, viability and function to ensure that it meets the necessary quality standards for clinical applications.

The Kosheeka CD8+ T cells are isolated from peripheral blood from healthy donor, using negative immunomagnetic selection.

Product Category	Homo Sapiens, Human
Product Type	Adherent population of cells
Derived From	Peripheral Blood
Cell Morphology	Round, light emitting cells
Culture & Growth Properties	Adherent population with elongated flap like structures
Passage No.	P2
Mycoplasma	Detected on demand
Hepatitis B	Negative
Hepatitis C	Negative
HIV-1	Negative
Aerobic & Anaerobic Bacteria	Negative after 7 days of culture
Positive for	CD8
Negative for	CD 34

These are the type of T cells, also known as CD8+ cells with the ability to regulate immune response in the body and fight against invading pathogens. They also regulate production of abnormal cells like cancer cells. There are several sources of cytotoxic T cells like bone marrow, peripheral blood and umbilical cord blood, which can be easily exploited to extract cells.

Bone marrow is the most potent source of cytotoxic T cells, where the immature T cells or naïve cells are produced. The choice of source depends upon your research demand, if your study requires immature T cells, bone marrow is the best match. However, if mature T cells, positive for CD8+ is required, they can be easily isolated from peripheral blood, without much ethical concerns.

Yes, cytotoxic T cells can be expanded in culture, known as T cell culture or lymphocyte culture. For expansion a specific T lymphocyte expansion media is suggested with either serum free option or with 2 % autologous human serum. The expansion of cytotoxic T cells is modified with the use of different cytokines, growth factors, co-stimulation as well as genetic modifications, depending upon number of applications.

Although, specific method for isolation of cytotoxic T cells depends upon the research applications, some of the general methods used for the isolation of T cells are density gradient centrifugation, magnetic bead separation, fluorescence activated cell sorting, etc. We generally isolate cells from a pool of healthy peripheral blood donor, obtained through leukapheresis and obtaining T lymphocytes from a pool of mononuclear cells; the cells are further purified using positive magnetic selection for different applications. However, we emphasize you to define your specifications and experimental requirements, so that we can offer the quality cells.

Each cell expresses a different subset of proteins, while CD8+ cell expresses CD8, the CD4+ T cell expresses the CD4 subset. Moreover, both the cells functions very differently, while CD8+ T cells are primarily involved in identifying foreign antigen and tumorigenic cell and promoting their phagocytosis and/or apoptosis, the CD4+ T cells are primarily involved in regulating immune response through secretion of a cocktail of cytokines and activating immune cells.

The CD8+ T cells have a specific receptor known as T cell receptor, which identifies a major histocompatibility complex formed with a particular foreign antigen. Upon identification, the TCR binds with the major histocompatibility complex on the cellular surface and activates intracellular signalling pathways, in order to initiate their phagocytosis and apoptosis.

The CD8+ T cells are activated upon recognition of specific major histocompatibility complexes presented on the surface of invading foreign antigen as well as abnormal cells. These antigens are presented to the T cells by specialized cells, called antigen-presenting cells (APCs), like dendritic cells. Once activated by the CD8+ cells undergo clonal expansion and differentiate into effector cells that can recognize and get rid of the targeted antigens.

The culture of CD8+ T cells have been extensively used in multiple research and development projects, including the development of immunotherapies for cancer and infectious diseases. The cells are also used in the study of autoimmune disorders, and the generation of engineered T cells for adoptive cellular therapies. Additionally, CD8+ T cells cultures can be used to test the efficacy and safety of drugs development and toxicity analysis.

Certain cells like CD8+ T cells are difficult to expand in the culture due to the requirement of addition of certain cocktail of growth factor or cytokines to promote cellular expansion. Additionally, these cytokines are highly sensitive and specific about their concentration as well as other optimum culture conditions; if not maintained properly, they can initiate cellular apoptosis. Moreover, these cells are also very sensitive about the culture hand, any wrong handling can create contamination in the culture.

The transportation of CD8+ T cells can either be in adherent form or in cryopreserved form in dry ice. Kosheeka transports CD8+ T cells with unique technology through which cells are being shipped at room temperature to maintain the viability and function of the cells during transit. We encourage proper quality control of the cells being shipped for viability, characterization and functional contribution.