Human Peripheral Blood-Derived Mononuclear Cells

General Description Specifications Certificate Of Analysis Technical Library

The peripheral blood-derived mononuclear cells are a cocktail of potent progenitors along with immune cells like NK cells, T cells, and B cells. Additionally, the cocktail is also a rich source of monocytes that are destined to be differentiated into macrophages and/or dendritic cells. The cells are isolated using the kit with the help of density gradient separation. All the red blood cells are thoroughly depleted by proprietary techniques with osmotic lysis. Although the cellular profiles of mononuclear cells are mostly donor-dependent, on average, they may contain around 50% T cells, 20% monocytes, 10% B cells, and 10% NK cells. The collection is from healthy donors, who are screened for infectious markers like HIV-1, Hepatitis B, and Hepatitis C. Each lot shall be provided with a Certificate of Analysis. Kosheeka is able to demand a viable population of freshly isolated mononuclear cells. These cells will be transported in HBSS along with 5 mM EDTA and 0.5% BSA. We recommend the use of any suitable hematopoietic growth medium from a reputed brand, along with a combination of growth factors/cytokines, depending upon the study requirements and applications.

Product Category	Homo Sapiens, Human
Product Type	Non-adherent suspension cells
Derived From	Peripheral Blood
Cell Morphology	Round, light-emitting cells
Culture & Growth Properties	Non-adherent population
Passage No.	P0
Mycoplasma	Detected on demand
Hepatitis B	Negative
Hepatitis C	Negative
HIV-1	Negative
Aerobic & Anaerobic Bacteria	Negative after 7 days of incubation
Positive for	CD34, CD45
Negative for	CD105

The Kosheeka is isolating a mononuclear fraction of cells from peripheral blood obtained from a healthy donor, after informed consent; using the density gradient centrifugation method.

The exact percentage of cells depends largely upon the donor demographics; however, on a generalized note, a mononuclear fraction contains lymphocytes including T-cells, B-cells, and Natural Killer cells along with monocytes and dendritic cells.

The CD34+ cells make up less than 1% of the population of peripheral blood.

The whole, unprocessed peripheral blood typically contains around 4-10 million mononuclear cells per ml.

Yes, you can expand mononuclear cells in the culture for a certain period; however, it is important to add certain types of cytokines in the culture to allow them to proliferate in the culture without differentiation.

No, the viability of the cells will not be affected if isolated from donors with diabetes or a diseased condition; however, the percentage population of cell types present in the mononuclear fraction can be affected.

The peripheral blood is collected from a healthy donor using apheresis. The mononuclear cell fraction is separated from the rest of the blood using a differential centrifugation technique; which is cultured using a suitable growth medium from any reputed brand. Post expansion, cells should be stimulated and cultivated for 3 days, using 1 ug/ml anti-CD3 and 2 ug/ml anti-CD28 antibodies.

Yes, please find attached the reference document.

The mononuclear cells isolated from peripheral blood or other potential sources are mostly used in Immunology, infection biology, hematology as well as cancer research. With optimum culture conditions, the average doubling time shall be between 20 hrs. However, it should be noted that

These mononuclear cells can be stored at -800c for six months with the usual cryostorage procedures for about six months. The same can be stored at -1960c for a longer period.