Human Plateable hepatocytes

Models for drug-to-drug interactions (DDI) with more clinical relevance are a serious concern for any new drug development project. The plateable hepatocytes are specialized primary liver cells, isolated from liver tissues of different species, depending upon customer requirements. These cells display a unique property of being able to adhere and grow on culture plates or […]

Available Formats:
Proliferating: ≥500000 viable cells shipped in appropriate growth medium (T25 Flask).
Cryopreserved: A cryogeneic vial containing ≥500000 cells

Cat. No.	Product Name
0605HU00-01	Plateable hepatocytes pooled

+ Product Sheet
+ MSDS
+ PI

General Description Specifications Certificate Of Analysis Technical Library

At Kosheeka, these cells are tested for high viability and healthy culture duration. The cells isolated at Kosheeka support the multiple potential of DDI, as they are induction-qualified. The cells are characterized by 3 major mechanisms:

- Transporter activity

- Enzyme activity

These cells are featured for their confluence monolayer and distinct morphology with a cobblestone appearance. Inducibility of enzyme activity for CYP3A4, CYP2B6, and CYP1A2. Basal metabolism for 8 CYPs, SULT, UGT, and aldehyde oxidase are analyzed for the product. These cells are pretested for long-term culture, spheroid formation, and 96-well compatibility.

Application

- Xenotransplantation

- Low clearance metabolism studies

- Disease modeling

- Xenotransplantation

- 3D cell culture

- Short-term cellular toxicity studies

Product Category:	Homo Sapiens, Human
Product Type:	Adherant cells
Drived From:	Liver tissues
Cell Morphology:	Cobblestone morphology with tight junctions
Culture and Growth Properties:	Adherent population of cells
Passage No:	P1
Mycoplasma:	Detected on demand
Hepatitis B:	Negative
Hepatitis C:	Negative
HIV-1:	Negative
Bacteria, yeast and other fungi:	Negative after 7 days of culture
Positive for:	Transporter qualified
Negative for:	CD34

The plateable are cultured in specialized media and cultured conditions, which support their optimum growth. The medium is also supplemented with growth factors and nutrients to maintain their liver-specific functions and viability.

Our QC has tested the product for multiple passages in the culture, up to passage 5. However, a number of passages depend upon the actual cultural conditions, media used, and handling.

Yes, commonly hepatocytes are cultured using William’s E medium with 10% FBS. The complete media is further supplemented with specific growth factors like hepatocyte growth factors, using an optimum concentration.

Yes, these plateable hepatocytes can be cryopreserved for long-term storage.

The hematopoietic culture media was supplemented with 20% FBS and 25 mM HEPES. No plating media is required when seeding spheroids.

These cells are required to be cultured on collagen coating for strong adherence.

Yes, the optimized protocols for the standard culture in the 6-well plate require around 5,000 cells per well. Plating more cells per well may create a problem, as fewer cells may be attached at higher densities.

In order to avoid cellular compaction in the pellet, and difficulties in resuspending the compactly placed cells; it is always recommended to use 50 ml round bottom tube for pelletting.

We will provide you a detailed Certificate of Analysis along with the products; however, if you want a raw data from us for the publication point of view, you may have to sign a non-disclosure agreement with us further confirming that you will mention Kosheeka to be your cell supplier; followed by which, we will be able to provide you all the raw data.

Yes, we can offer you all kinds of customizations as per your demand.