The human umbilical cord blood derived CD34+ cells are an enriched population of stem cells isolated from the buffy coat containing mononuclear cells. CD34 is a receptor protein that is expressed on the surface of stem cells, thus isolation of CD34 enriched population of cells provides user with a pure population of stem cells, which […]
Available formats: ≥ 2.5 x 106/ ml. cryopreserved in suitable medium
The human umbilical cord blood derived CD34+ cells are an enriched population of stem cells isolated from the buffy coat containing mononuclear cells. CD34 is a receptor protein that is expressed on the surface of stem cells, thus isolation of CD34 enriched population of cells provides user with a pure population of stem cells, which can further be used for research and other applications. The CD34+ positive cells can potentially be differentiated into a variety of cell populations, including cells of hematopoietic origin like blood cells and cells of mesenchymal origin like muscle cells, bone cells, fat cells etc. The separation and expansion of CD34+ cells is a critically important step to maintain sterility, specificity, purification and regeneration capacity of isolated populations of cells. Kosheeka recommends the use of positive magnetic selection techniques for the isolation of these cells. It involves the use of magnetic beads coated with specific antibodies for the receptors and passage through the magnetic field to pull CD34+ cells towards the magnet, while the CD34- cells are separated. Human umbilical cord blood is considered to be the potent source of CD34+ cells, accounting for less than 1% of the circulating T cells. The cells are characterized specifically for the pure population CD34 markers. Kosheeka suggests use of a specific growth medium containing certain growth factors, a cocktail of stem cell factors like IL-3 and erythropoietin to the culture dish. However, it is very important to maintain sterile culture conditions and establish proper safety protocols, while working with the culture of the CD34 population to avoid contamination.
Product Category
Homo Sapiens, Human
Product Type
Non-adherent population of cells in suspension
Derived From
Human umbilical cord blood
Cell Morphology
Round, light emitting cells with variable morphology
Culture & Growth Properties
Non-adherent population
Passage No.
P1
Mycoplasma
Detected on demand
Hepatitis B
Negative
Hepatitis C
Negative
HIV-1
Negative
Aerobic & Anaerobic Bacteria
Negative after 7 days of culture
Positive for
CD 34
Negative for
CD 105
Cord blood derived CD34+ cell culture is a culture of enriched population of CD34+ cells, isolated from the mononuclear fraction through differential centrifugation process on cord blood. Out of all the available sources of these cells like peripheral blood, bone marrow etc., the cord blood is acknowledged to be the most potent source, with highest recovery of CD34+ cells. The cells isolated through positive magnetic bead separation are the pure population with more than 90% positivity rate for CD34 antibodies.
The pure population of cord blood derived CD34 positive cells is an enriched fraction of stem cells that are potent to be differentiated into cells of neuronal origin, bone cells and fat cells as well. These cells are identified to be effective tools for therapeutic application in blood borne disorders and certain types of blood cancer. Various studies are ongoing requiring quality isolation of these cells for better understanding.
No, magnetic separation is one of the most standardized methods established for the isolation and purification of CD34+ population with higher recovery and potency. Apart from the same, however, various other methods are being established like fluorescence activated cell sorting.
The specific culture medium for hematopoietic culture of mononuclear population is suggested with the addition of specific growth factors in optimum concentration to promote culture of CD34+ cells. Additionally, certain cytokines like IL-3, stem cell factors along with erythropoietin are also suggested for expansion of CD34 positive cells without differentiation. The culture medium from any reputed supplier can be used for the same.
The number of passages for the expansion of CD34+ population depends upon many factors like the starting of the cell population, culture condition, and the purpose of the study. In general, it is to be noted that the CD34+ population has a limited life span in the culture, and their ability to proliferate and differentiate into different cell types decreases with every passage. With optimum cell culture conditions, these cells can be expanded up to passage 5-7. One should also note that long term culture of CD34 positive population is associated with changes in the gene expression profile and loss of stemness; further affecting downstream application. Thus, Kosheeka recommends the careful monitoring of the passages with appropriate numbers to ensure their sustainability for the intended applications.
The cultured cord blood derived cells can be used for a variety of downstream applications, including differentiation into various blood cell lineages, in vitro screening studies, clinical and therapeutic applications in certain cancers as well as autoimmune disorders, etc. A huge number of studies are trying to incorporate exploitation of the CD34 positive population for gene therapies as well.
The culture conditions for CD34+ cells typically involves use of optimum growth medium from any reputed manufacturer with optimum use of specific growth factors and cytokines like stem cell growth factors, IL-3, erythropoietin and thrombopoietin depending upon the study requirements. The cells are usually cultured till passage 5 without much differentiation at 370c in a humidified atmosphere with 5% CO2.
Yes, serum free medium can be used for the culture and expansion of these cells. These media can be used to reduce the risk of xenogeneic transmission during clinical application and further ensure result reproducibility.
Yes, CD34+ cells isolated from the human umbilical cord blood can be cryopreserved for future applications with standard method of cryopreservation at -1960c.
Yes, these cells can be differentiated while in the culture into different blood cell lineages using specific differentiation protocol. To differentiate into red blood cells, a medium containing erythropoietin growth factors can be used, while differentiation into white blood cells is accomplished by a medium containing granulocyte colony-stimulating factor (G-CSF) or granulocyte macrophage colony-stimulating factor (GM-CSF).
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