Human Umbilical Cord Blood Derived Mononuclear cells
The human umbilical cord blood derived mononuclear cells is a pure population of precursor cells, isolated from cord blood, shortly after birth. The population contains a mixture of progenitors including lymphocytes, monocytes and stem cells, and are widely studied for their therapeutic potential and immune regulation properties. The cryopreserved as well as fresh batch of […]
Available formats: ≥ 2.5 x 107/ ml. cryopreserved in suitable medium
The human umbilical cord blood derived mononuclear cells is a pure population of precursor cells, isolated from cord blood, shortly after birth. The population contains a mixture of progenitors including lymphocytes, monocytes and stem cells, and are widely studied for their therapeutic potential and immune regulation properties. The cryopreserved as well as fresh batch of mononuclear cells is isolated from cord blood via density gradient separation technique. We are separating with a single spin kit to separate erythrocytes and granulocytes further producing a more stable product.Kosheeka supplies a pool of mononuclear fraction from cord blood both in fresh isolation as well as in cryopreserved format, depending upon the requirements. The product can also be customized depending upon the study parameters like single donor, pooled, with variable cell count per vial. It is also possible to culture and expand these cells further to obtain a choice of cells like mesenchymal stem cells or lymphocytes etc. depending upon the study requirements.The pure population of mononuclear cells have been shown to have various therapeutic applications, including the treatment of autoimmune diseases, like multiple sclerosis, rheumatoid arthritis etc, along with tissue repair and regeneration. Thus, upon demand Kosheeka is able to supply pure population of freshly isolated mononuclear cells from umbilical cord blood in a suspended form in HBSS with 5mM EDTA and 0.5% BSA.The characterization of cord blood derived mononuclear cells is important to understand their potency, cellular composition as well as functional properties. With the help of various advanced techniques like viability analysis, flow cytometry, complete blood count etc. the researchers can gain a comprehensive understanding of mononuclear population and tailor their use for specific applications.
Product Category
Homo Sapiens,Human
Product Type
Non-adherent population of cells in suspension
Derived From
Human umbilical cord blood
Cell Morphology
Round, light emitting cells with variable morphology
Culture & Growth Properties
Non-adherent population
Passage No.
P1
Mycoplasma
Detected on demand
Hepatitis B
Negative
Hepatitis C
Negative
HIV-1
Negative
Aerobic & Anaerobic Bacteria
Negative after 7 days of culture
Positive for
CD 34, CD45
Negative for
CD 105
The optimum culture condition depends upon your area of research and experimental requirements. In general, CBMNCs are required to be cultured in specific media along a cocktail of growth factors to promote expansion of cells of interest. Certain combination of growth factors and cytokines can also be supplemented along with media to support proliferation of cells of interest without being differentiated, such as T cells, lymphocytes, monocytes, B cells etc.
Yes, CBMNCs can be cultured in serum free condition for reducing the xenogeneic transmission of contaminants; especially if cells are used for clinical applications. Along with the same, serum free culture medium can also improve reproducibility of cell recovery, viability and proliferation capacity. Instead of serum free medium, an autologous human serum can also be a good option for optimum cell growth and expansion.
The CBMNCs can be differentiated into specific cell types using various differentiation protocols that further mimics the in vivo environment. Thus, to differentiate CBMNCs to red blood cells, an erythropoietin can be added with optimum concentration, while the same cells can be differentiated to dendritic cells with the addition of GM-CSF and interleukin 4 to the culture medium.
Due to increased potency, CBMNCs have a wide range of potential downstream applications, including cell-based therapies for immune disorders and certain types of cancer. These cells can also be used for research and development, including in vitro screening, in vivo studies, toxicity analysis etc.
Some common challenges encountered during culturing CBMNCs include low cell yield, reduced proliferation capacity, limited lifespan in cell culture, and batch to batch variation. Since these cells are highly sensitive to culture conditions, Kosheeka recommends use of optimum media with specific concentration of growth factors and cytokines. Moreover, other cultural parameters like temperature, pH, oxygen levels etc. should also be checked intermittently for cell viability and functions.
It is important to routinely monitor cell viability, rate of proliferation, and differentiation of isolated CBMNCs using combinational protocols like flow cytometry, gene expression analysis, as well as other functional assays. Kosheeka suggests strict adherence to good laboratory practices and standard operating procedures, which can further minimize the risk of contamination and ensure reproducibility.
The genetic modification of CBMNCs is required in certain studies and can be accomplished with the help of various advanced techniques like viral transduction, electroporation, CRISPR/Cas 9 genome editing, etc. The gene modification can be used to add or delete particular gene expression for understanding molecular mechanisms.
The primary challenge during cell transportation is the cell viability, due to lack of optimum condition. Thus the use of specific antibiotics to reduce contamination and comply with the regulatory requirements to ship biological products is necessary.
It is recommended to use appropriate shipping conditions and packaging material to facilitate transportation of good quality cells. Some of the best practices include the use of insulated containers, ice packs, absorbent material to maintain the temperature and install proper shock absorbance techniques during transportation. Moreover, cells are recommended to be transported as quickly as possible to minimize the time spent during transit. Kosheeka recommends use of appropriate transport conditions that are validated to ensure that cells remain viable during shipment.
Upon receipt of cells, certain common methods can be employed to ensure successful transportation of cells that include visual inspection of cells, like checking changes in cell morphology, colour, and using assays that measure metabolic activity like MTT assays.
The shipment of the cells is regulated by various national and international agencies like the international air transport association (IATA). These institutions have proposed various guidelines regarding packaging, labelling and documentation of biological material, as well as restrictions on the shipment of hazardous or infectious materials. Thus, it is important to be aware of and comply with relevant regulations when shipping cells.
The choice of method for cellular dissociation depends upon the source of cells and experimental requirements. In general, enzymatic digestion is often suggested and preferred for dissociating blood cells.
The quality of dissociated cells can be assessed using a variety of methods, including flow analysis, cellular viability, MTT analysis etc.
There are several factors that can affect the success rate of cellular dissociation, such as the choice of dissociation enzyme used, at what concentration the dissociation enzyme is used, the duration of the dissociation process along with type and age of cells in culture.
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