Human Peripheral Blood-Derived Mesenchymal Stem Cells

Available Formats:

Proliferating: ≥500000 viable cells shipped in appropriate growth medium (T25 Flask).
Cryopreserved: A cryogeneic vial containing ≥500000 cells

General Description Specifications Certificate Of Analysis Technical Library

Human peripheral blood-derived mesenchymal stem cells are a type of stem cells obtained from the peripheral blood of healthy donors. These cells are the multipotent population with the ability to differentiate into cells of various tissue origin, like bone, cartilage, fat, and muscle cells. The population of cells is currently highly exploited in the field of regenerative medicine. The cells are being investigated for use in various therapeutic applications, including bone and cartilage repair, wound healing, and treatment of inflammatory and autoimmune diseases. One of the advantages of PB-MSCs is their ease of isolation from a non-invasive source, as compared to other counterparts like bone marrow. Their higher proliferation rate makes them a valuable tool for other clinical applications as well. Kosheeka is isolating a pure population of peripheral blood-derived mesenchymal stem cells from a mononuclear fraction of peripheral blood, using a process called density gradient centrifugation. Once, isolated these cells can further be expanded in culture and characterized for various markers like CD105, CD 29, CD73, etc. On-demand, Kosheeka can also help to purely isolate mesenchymal stem cells through the negative selection method. These cells demonstrate a unique ability to differentiate into adipogenic, chondrogenic, and osteogenic lineages; when cultured in a definite differentiation medium. All cells test negative for mycoplasma, bacteria, yeast, and fungi. All our donors are screened for infectious diseases, and a detailed certificate of analysis is provided for each cell lot purchased.

Product Category	Homo Sapiens, Human
Product Type	Adherent, anchorage-dependent population
Derived From	Peripheral Blood
Cell Morphology	Spindle-shaped, fibroblasts-like morphology
Culture & Growth Properties	Doubling time of approximately 20hrs.
Passage No.	P0
Mycoplasma	Detected on demand
Hepatitis B	Negative
Hepatitis C	Negative
HIV-1	Negative
HBsAg	Negative
HBcAg	Negative
Aerobic & Anaerobic Bacteria	Negative after 7 days of incubation
Positive for	CD105, CD73, CD29
Negative for	CD34, CD45, HLA-DR

Kosheeka human peripheral blood-derived mesenchymal stem cells are isolated from the mononuclear fraction of peripheral blood through differential centrifugation. The fraction is then expanded in culture with suitable growth media to promote the proliferation of mesenchymal stem cells.

The mesenchymal stem cells are isolated from different other sources like the umbilical cord blood, umbilical cord Wharton’s jelly, bone marrow, and adipose tissue. Apart from the primary sources given herewith, mesenchymal stem cells can also be isolated from skin, cartilages, synovial fluid, bone

Various studies have proven multipotential differentiation abilities of mesenchymal stem cells. It has been confirmed that mesenchymal stem cells can differentiate into cells of ectodermal origin like skin, neurons, glial cells, etc. They can also be differentiated into cells of mesodermal origin, like bone, cartilages, fat and muscle cells. While some studies have also confirmed that mesenchymal stem cells can also be differentiated into certain cells of endodermal origin like hepatocytes and pancreatic beta cells.

The international society of cellular therapy has listed certain unique characteristics of mesenchymal stem cells, like their anchorage dependency. These cells are only grown when adhered to the polypropylene surface of the flask. They always test positive for certain specific surface markers like CD105, CD73 and CD90, while lacking expression for certain other surface antibodies like CD45, CD34, CD19 and HLA-DR.

A kind of media is suggested depending upon the application of mesenchymal stem cells. For therapeutic application, generally serum-free media is preferred to avoid xenogeneic transfer of potential contaminants, while for research and development purpose, mesenchymal stem cells can be cultured in DMEM+F12, obtained from any reputed manufacturer, along with 10% fetal bovine serum.

Mesenchymal stem cells at a confluency of 60-70% can be successfully transported, by feeling the media till the neck of the flask and replacing vented cap with the non-vented one to avoid gaseous exchange during transportation. Live mesenchymal stem cells can be transported within 24 hrs.

Once, you receive a flask of mesenchymal stem cells, you should immediately observe the flask under the microscope, for any signs of contamination and change in the morphology. If everything is ok, immediately reduce the amount of medium. Generally, for a T75 flask, 10 ml of media is recommended. Replace the non-vented cap with a vented cap and keep the flask in an incubator for further stabilization.