The 3D Cell Culture & Tissue Engineering
Ever since a technique of growing cells in an artificial environment has been established, it has evolved with no leaps and bounds. Today, different techniques of cell culture have been routinely used to understand different properties of cells and their applications. Amongst them, 3D cell culture is currently trending for its advanced and convenient features available as compared to their alternatives. Moreover, 3D cell culture is expected to solve some mysteries related to in vitro organ cultures, opening altogether new dimensions of organ replacement techniques as compared to conventional organ transplants.
At Kosheeka we are advancing our knowledge and expertise to support this exclusive journey of in vitro organ culture.
As all of us may already be aware, currently different techniques available for 3D cell culture; while each of them offers different positives and a few negatives. Before moving ahead to understand them, it is always better to first compare 3D cell culture with its conventional counterpart, the 2D technique.
3D cell culture technique is vastly privileged to facilitate cellular differentiation and tissue organization using micro-assembled, custom-designed structures, supported by a complex microenvironment. Many studies have vetoed that cells in 3D culture are very sensitive toward morphological as well as physiological changes; which not only influence genetic expression but also enhance cellular communication.
With the help of 3D cell culture, researchers can grow two different cellular populations together, which are known as cocultures and can exactly reproduce the cellular functions observed within a tissue.
Thus, unlike 2D culture, 3D culture is a relatively new technique and requires a lot of understanding, expertise, and supportive accessories like different 3D culture matrices. However, for various applications 3D cell culture is a more satisfactory model for mimicking in vivo cell behaviors and organizations. While assembling multi-layered 3D cell culture requires optimum supporting microenvironment influencing cellular morphology, and differentiation potential to the great extent.