Dog hepatocytes plateable

Available formats:
Proliferating ≥500000 viable cells shipped in appropriate growth medium (T25 Flask).
Cryopreserved: A cryogeneic vial containing ≥500000 cells

Dog mesenchymal stem cells adipose tissue

Available formats:
Proliferating ≥500000 viable cells shipped in appropriate growth medium (T25 Flask).
Cryopreserved: A cryogeneic vial containing ≥500000 cells

Horse mesenchymal stem cells-adipose tissue

Available formats:
Proliferating ≥500000 viable cells shipped in appropriate growth medium (T25 Flask).
Cryopreserved: A cryogeneic vial containing ≥500000 cells

Horse peripheral blood mononuclear cells

Available formats:
Proliferating ≥500000 viable cells shipped in appropriate growth medium (T25 Flask).
Cryopreserved: A cryogeneic vial containing ≥500000 cells

Human Aortic Endothelial Cells

Aortic smooth muscle cells constitute a major part of arteries and are responsible for maintain vascular integrity against the pulses from the heart. These cells are used to study various diseases such as arteriolosclerosis and arterial disease as well as in vascular wound healing, tissue remodeling and drug screening applications.

Human Aortic Smooth Muscle Cells

Cardiac fibroblasts form the major part of heart tissue. They produce connective tissue which maintains the extracellular matrix of the heart. They are essential in studying proper heart function and physilogy. Research using these cells includes neointimal formation, tissue remodeling, myocardial and vascular repair, toxicology and drug screening, genetic therapy, extracellular repair, growth factors and cytokines.

Human Bronchial Fibroblasts

Isolated from the distal portion of lung tissue these cells are essential in studying lung function. wound healing, airway inflammation as well as progression of pulmonary diseases such as asthama and COPD. They can also be used for toxicity analyisis and drug screening.

Human Cardiac Fibroblasts

Pericardial fibroblasts are major components of the pericardium layer that acts as protective sac for the heart. These cells produce collagens to give the heart strucutral support and lubrication. They are used to study progression of sclerosing pericarditis and drug screening puporsoses.

Human Cardiomyocytes

Isolated from the ventricles of the adult heart. These cells are qualified for the in vitro research on cardiac diseases as well as for pharmacological studies. The HCM can be cultured for as long as 10 population doublings, and hence are suitable for long term experiments.

Human Islet Beta Cells

Found in the pancreas these cells are responsible for the production of insulin and amylin. These cells are used for drug discovery and research related to diabetes, beta cell replacement, genetic therapies.

Human Islets Beta cells

Available Formats:
       Proliferating: ≥500000 viable cells shipped in appropriate growth medium (T25 Flask).
       Cryopreserved: A cryogeneic vial containing ≥500000 cells

Human Kidney Fibroblasts

Isolated from human kidney biopsies, these fibroblasts are adherent-dependent cells cultured on gelatin-precoated flasks and are transported in adherent form only. These cells can be expanded up to 3-4 population doublings. These cells are tested for negative expression of Von Willibrand Factor and factor VIII and alpha-smooth muscle actin. These cells are used for in vitro assays and toxicological studies. 

Human Mast cells

Part of the immune system, Mast cells help fight infections and are responsible for creating allergic reactions. They can be used to study specific antigens and antibody responses.

Human Microvascular Endothelial Cells

Found in the smallest vessels of the vascular system, like capillaries these cells are important for maintaining vascular homeostasis. They are used for drug screening, toxicity analysis as well research on inflammation, cancer and intercellular communication.

Human Pericardial Fibroblasts

Isolated from the lungs, pulmonary fibroblasts are crucial in maintain alveolar integrity and repair injury. They are gennerally used to study progression of lung disorders and infections. Research is ongoing to use these cells for lung reconstruction and as a diagnostic method for early detection for lung cancer. Moreover, they are alsi used for drug screening especially in the case of pulmonary infections.

Human Plateable hepatocytes

Models for drug-to-drug interactions (DDI) with more clinical relevance are a serious concern for any new drug development project. The plateable hepatocytes are specialized primary liver cells, isolated from liver tissues of different species, depending upon customer requirements. These cells display a unique property of being able to adhere and grow on culture plates or dishes. These cells are used as indispensable tools for researchers in drug development, toxicology, disease modeling, as well as liver-related studies.

At Kosheeka, these cells are tested for high viability and healthy culture duration. The cells isolated at Kosheeka support the multiple potential of DDI, as they are induction-qualified. The cells are characterized by 3 major mechanisms:

-          Transporter activity

-          Enzyme activity

These cells are featured for their confluence monolayer and distinct morphology with a cobblestone appearance. Inducibility of enzyme activity for CYP3A4, CYP2B6, and CYP1A2. Basal metabolism for 8 CYPs, SULT, UGT, and aldehyde oxidase are analyzed for the product. These cells are pretested for long-term culture, spheroid formation, and 96-well compatibility.

Application

-          Xenotransplantation

-          Low clearance metabolism studies

-          Disease modeling

-          Xenotransplantation

-          3D cell culture

-          Short-term cellular toxicity studies

Human Pleatable Hepatocytes Pooled

Part of the immune system, Mast cells help fight infections and are responsible for creating allergic reactions. They can be used to study specific antigens and antibody responses.

Human Pulmonary Fibroblasts

The  major organs of the respiratory system are lungs, which helps provide the body with a continuous supply of oxygen. The main role of lungs is to bring in air from the atmosphere and pass oxygen into the bloodstream. From there, it circulates to the rest of the body.

Fibroblasts are the most abundant cell type in lung interstitium. They do resemble ordinary fibroblasts but have certain distinguishing features, such as long branching processes and gap junctions. The main function of HPF (Human Pulmonary Fibroblasts) is to produce type III collagen, elastin, and proteoglycans in the extracellular matrix of the alveolar septa. Pulmonary fibroblasts (PF) are responsible to carry out the repair and remodelling processes following an injury. There is a controlled accumulation of the fibroblasts to the sites of inflammation which is crucial for effective tissue repair after injury. Abnormal tissue function occurs when there is an inadequate or an excessive accumulation of fibroblasts. For instance, the excess proliferation of fibroblasts contributes to the adventitial thickening observed during the development of hypoxia-induced pulmonary hypertension.

Lung fibroblasts play an important role in airway inflammation and airway remodelling. In pharmaceutical development they can be a target for asthma medications. Fibroblasts are the most abundant cell type in lung interstitium. Pulmonary fibroblasts do resemble ordinary fibroblasts but have some distinguishing features, for example, they have long branching processes and gap junctions. Their main function is production of type III collagen, elastin, and proteoglycans of the extracellular matrix of the alveolar septa.

The most abundant cells in the pulmonary interstitium are Human Lung Fibroblasts (HLFs). These mesenchymal cells produce the extracellular matrix (ECM) giving the lungs its structural integrity, and are best known for their critical role in wound healing. In normal wound healing, fibroblasts, often under the direction of the master profibrotic cytokine transforming growth factor β (TGF-β), differentiate into contractile myofibroblasts that are characterized by the presence of cytoplasmic stress fibres and a focal adhesion complex, the expression of α-smooth muscle actin (α- SMA) and calponin, and enhanced synthesis of ECM proteins such as collagen and fibronectin.

Extracellular matrix (ECM) consists of a highly dynamic complex of fibrous proteins, glycoproteins, and proteoglycans that composes the noncellular aspect of tissues and varies in composition according to its tissue localization and physiological circumstances. While providing structural integrity, ECM also delivers important spatial and contextual cues to drive cellular phenotypes. The ECM in the lungs is typically divided into two basic compartments: basement membranes and the interstitial spaces. The thin and specialized layers of ECM found under all epithelial and endothelial cell layers are called basement membranes, whereas interstitial spaces form the parenchyma of the lung. Within the lung interstitium, are the resident fibroblasts which are the most commonly identified cells and are mainly responsible for ECM production; they also function as an effector cells during injury repair.

Primary Lung Fibroblast, normal, Human Lung Fibroblast (HLF) is a cell line that is ideal culture model for potential diagnostic methods for the early detection of lung cancer cells, reconstruction studies, and advancement of cancer research. Primary lung fibroblasts are an essential tool for the profiling of normal human lung fibroblasts and serve as a model system for the study of lung disorders and infections, lung reconstruction studies, and advancement of cancer research.

Human Pulmonary Small Airway Epithelial Cells

Isolated from the spleen, Human Splenic endothelial cells are being increasingly recognised for their role in the immune system. Apart from being an interface between the the spleen tissue and blood, these cells are also responsible for the growth of dendritic cells. Moreover, researchers have studied the endotheliel cell-macrophage interactions to show that these cells have an important role in the phagocyte system. These cells can also be used for toxicity analysis and drug screening.

Human Splenic Endothelial Cells

Isolated from the spleen, these cells have MSC like charactersitcs. In the spleen they are repsonsible for the creation of a non-rigid extracellular matrix. They also contribute to growth of NK cells and bioactive IL-15. They can be used in various studies involving cell manipulations such as genetic transfers. They are also used for IVD and drug screening purposes.

Human Splenic Fibroblasts

Human splenic fibroblasts are derived from the fibroblast cells found in the spleen, an important organ of the immune system. These fibroblasts play a crucial role in the structural support and maintenance of the spleen tissue. Human splenic fibroblasts are utilized in various research areas related to the immune system and the spleen.These cells are used to study the interactions between immune cells and the spleen microenvironment. They provide a valuable model for investigating immune responses, immune cell migration, and the immune regulatory functions of the spleen.

Kidney Fibroblasts

Available formats:
Proliferating ≥500000 viable cells shipped in appropriate growth medium (T25 Flask).
Cryopreserved: A cryogeneic vial containing ≥500000 cells

Liver Hepatocytes plateable

Available formats:
Proliferating ≥500000 viable cells shipped in appropriate growth medium (T25 Flask).
Cryopreserved: A cryogeneic vial containing ≥500000 cells

Lung alveolar cells

Available formats:
Proliferating ≥500000 viable cells shipped in appropriate growth medium (T25 Flask).
Cryopreserved: A cryogeneic vial containing ≥500000 cells