Transforming Research with Customized Cell Culture Solutions
In the realm of cellular biology and biomedical research, “One-size fits all” doesn’t hold true. It demands precise and adaptable cell culture solutions for studying cellular metabolism or disease mechanisms.
In this article, we will discuss how customized cell culture solutions are beneficial and how they are transforming the landscape of scientific research. Our customized cell culture services:
Primary Cell Isolation and Culture
We understand the importance of physiologically relevant models, we isolate and culture various primary cells from a variety of tissues from human and animal sources. We use our optimized robust protocols to maintain cell viability and functionality of primary cells such as peripheral blood mononuclear cells (PBMCs), fibroblasts, mesenchymal stem cells, epithelial cells, endothelial cells, microsomes, S9 fractions, skin cells (keratinocytes, melanocytes) etc. These primary cells maintain the signature characteristics to obtain accurate, reliable and precise experimental outcomes.
Stem Cells
We develop different types of stem cells and differentiate these stem cells towards various lineages. Mesenchymal stem cells from various sources like umbilical cord, adipose tissue, bone marrow etc.
Cell Co-culture Systems
Various primary cell cultures and cell lines play a significant role in the pharmaceutical industry. Conventional two-dimensional (2D) cultures fail to mimic the physiological in vivo conditions due to lack of cell-cell interactions and three-dimensional orientations. Three-dimensional culture methods and co-culture systems recapitulate the physiological in vivo conditions. We design co-culture systems to study cell-cell interactions. Epithelial-mesenchymal co-cultures to study cancer metastasis. Immune cell co-cultures to study immune responses and inflammations. Other customized co-cultures can also be designed as per customer’s experimental requirements. These ready-to-use cell co-culture systems are highly functional and amenable to high-content imaging and high-throughput screenings.
Cell Expansion and Banking
To support large-scale cell productions, we offer cell expansion, cryopreservation and banking services. Our state-of-the-art facilities are equipped to scale up cell cultures to meet your production requirements, from milliliter to bioreactor volumes. Additionally, we provide cryopreservation and cell banking services to safeguard your valuable cell lines and ensure their availability for future research and development.
Exosomes
Exosomes are nanosized vesicles (30-150 nanometers) secreted by various cell types in an extracellular environment. These exosomes are rich in proteins, RNA and DNA and carry various cell signaling molecules. Exosomes are isolated from spent culture media of in vitro cell cultures. Exosomes serve as prognostic and diagnostic biomarkers for various diseases. Exosomes hold therapeutic potential for multiple diseases like neurological diseases, diabetes etc due to their tissue repair and immunomodulatory potential. We provide exosomes from various cell sources as per customers’ experimental requirements. Exosomes are of high quality and well characterized for various research purposes.
Regenerative Medicine and Tissue Engineering
We provide customized solutions for regenerative medicine; we seed cells in customized scaffolds/ extracellular matrices as per experimental requirements.
State-of-the-Art Laboratory
Our more than 5000 square feet BSL1 and BSL2 laboratories are well equipped with state-of-art equipment to support cutting edge research. We are continuously upgrading to provide state-of-art services.
- Incubators and shakers for adherent and suspension cell culture
- Closed Bioreactor systems
- Automated and semi-automated cell-counters, plate readers
- Microbial studies
- Gene expression studies
- Flow Cytometry
- Diverse spent media analysis techniques
Certifications and Compliance
Kosheeka’s quality management system (QMS) is compliant with ISO 9001 and GMP. Our research projects, protocols and workflows are fully compliant with ISO 9001. To know more about our QMS and applications of our products and services please visit our website.
Quality Control and Characterization
To ensure the highest standard of quality, we perform comprehensive cell characterization and functionality studies. This includes mycoplasma testing, cell morphology analysis, cell line authentications e.g. short tandem repeat (STR), karyotyping, and molecular and functional analyses. Our quality control teams ensure delivery of contamination-free, functional and genetically stable cells. We adhere to stringent quality control measures to ensure the purity, viability, and functionality of all cell cultures and reagents.
Technical Support and Consultation
Our commitment towards customer satisfaction, we offer expert technical support to assist in designing optimized experimental plans. Our expert team of scientists assist in cell culture techniques, troubleshooting and data analysis. By collaborating with us for cell culture requirements, customers will gain access to a wealth of expertise to ensure seamless research outcomes.
At Kosheeka, a branch of the Advancells Group, we are committed to advancing the field of cell biology through our specialized and customized cell culture services. We offer tailored cell culture services to our clients in research, drug discovery, and clinical applications. Our services comprise a diverse range of primary cell lines, ensuring high-quality and robust protocols to accelerate scientific discoveries.
Frequently Asked Questions
What Types of Primary Cells are Isolated and Cultured by Kosheeka?
At Kosheeka, we isolate and culture various types of primary cells from human and animal sources. This includes Peripheral Blood Mononuclear Cells (PBMCs), fibroblasts, Mesenchymal Stem Cells, Epithelial Cells, Endothelial Cells, Microsomes, S9 fractions, and skin cells such as keratinocytes and melanocytes. Our optimised robust protocols ensure high cell viability and functionality.
What Stem Cell Services are Offered by Kosheeka?
We develop various types of stem cells from different tissue sources, and can differentiate them into various lineages. We provide mesenchymal stem cells from umbilical cord, bone marrow, adipose tissue, dental pulp etc.
How We Develop Co-Culture Systems?
We develop co-culture systems to mimic in vivo conditions and to study cell-cell interactions. This includes epithelial-mesenchymal co-cultures for cancer metastasis studies, immune cells co-cultures for immune and inflammation studies. These systems maintain the functionality and are amenable to high-content imaging and high-throughput screenings.
How Kosheeka Assists in Large-Scale Cell Production?
We offer cell expansion, cryopreservation and cell-banking services. Our facilities support scale-up of cell production needs, up to bioreactors volumes. We also assist in cell banking to safeguard valuable cell lines for future research and development.
What are Exosomes? How Kosheeka Provides Customized Exosomes to Support Various Research Projects?
Exosomes are nano-vesicles rich in proteins, RNA, and DNA, secreted by all cell types extracellularly. They serve as prognostic and diagnostic biomarkers, and hold therapeutic potential for diseases like neurological, metabolic and various other diseases. We provide high-quality, well-characterized exosomes from various cell sources customized as per research requirements.
What Facilities and Equipment does Kosheeka’s Laboratory Have?
Our 5000+ square feet BSL1 and BSL2 laboratories are equipped with state-of-the-art equipment including:
- Incubators and shakers for adherent and suspension cell cultures
- Closed bioreactor systems
- Automated and semi-automated cell counters and plate readers
- Facilities for microbial studies, gene expression studies, and flow cytometry
- Diverse techniques for spent media analysis
What Certifications and Compliance does Kosheeka Maintain?
Kosheeka’s quality management system (QMS) is compliant with ISO 9001 and GMP standards. Our research projects, protocols, and workflows adhere to these stringent guidelines to ensure high-quality products and services.
How does Kosheeka ensure Quality Control and Characterization?
We perform comprehensive cell characterization and functionality studies, including mycoplasma testing, cell morphology analysis, cell line authentication (e.g., short tandem repeat (STR) analysis), karyotyping, and molecular and functional analyses. Our quality control measures ensure the purity, viability, and functionality of all cell cultures and reagents.
Does Kosheeka provide Technical Support and Consultation?
We offer expert technical support to help design optimized experimental plans. Our team of scientists (Ph.D.-level scientists pool) assists with cell culture techniques, troubleshooting, and data analysis, ensuring seamless research outcomes for our clients.
For more information about our customized cell culture services and to learn how we can support your research needs, please contact us or visit our website.
