Amniotic Epithelial cells
Normal Human Amniotic Epithelial Cells are present in the outer lining of the umbilical cord and are isolated using suitable explant techniques. These cells are predominantly found in the amniotic membrane, the cord’s outer connective tissue, primarily responsible for cushioning and nourishment of the developing fetus. The anchorage-dependent cell displays characteristic fibroblasts like morphology, similar to that of mesenchymal stem cells. However, these cells are easily distinguished from the MSCs due to the presence of distinct surface markers and intracellular proteins.
At Kosheeka, we assure that our proliferating epithelial cells can be expanded for up to 15 population doubling. However, optimum culture conditions and expert handling play a crucial role in replicating the same at your facility. Rigorous quality control procedures are in place to certify that each batch of epithelial cells is negative for mycoplasma, bacteria and fungi. In addition to the same, we evaluate these cells for the presence of surface markers like CD46 and E-Cadherin as positive markers and CD105 as negative markers. We also examine the presence of intracellular proteins like cytokeratin and collagen I. However, this is not done for every batch. Upon specific requests assessments for these intracellular proteins and infectious markers like HIV, HCV etc. can be conducted.
In order to ensure complete transparency and confidence in our products we share Certificate of Analysis with each shipment; which provides comprehensive quality control information to our customers.